3x flag peptide (Thermo Fisher)

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Thermo Fisher 3x flag peptide
3x Flag Peptide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3x+flag+peptide/3x+flag+peptide/pm40592880-339-7-10
Average 90 stars, based on 1 article reviews

3x flag peptide - by Bioz Stars, 2026-09

90/100 stars

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Incubation:

Article Title: Cryo-EM reveals the architecture of the PELP1-WDR18 molecular scaffold
Article Snippet: Remaining 15 μL was transferred to a SigmaPrep spin column (Sigma) and used for native elution of protein using 3X FLAG peptide. .. 15 μL of 300 μg/mL 3X FLAG peptide (Pierce) in high salt wash buffer was incubated with 15 μL of protein-bound anti-FLAG gel for 30 minutes at 4 °C and on a nutator. ..

Article Title: Cryo-EM reveals the architecture of the PELP1-WDR18 molecular scaffold.
Article Snippet: Remaining 15 μL was transferred to a SigmaPrep spin column (Sigma) and used for native elution of protein using 3X FLAG peptide. .. 15 μL of 300 μg/mL 3X FLAG peptide (Pierce) in high salt wash buffer was incubated with 15 μL of protein-bound anti-FLAG gel for 30minutes at 4 °C and on a nutator. ..

Lysis:

Article Title: Mechanisms of MCL-1 Protein Stability Induced by MCL-1 Antagonists in B-Cell Malignancies
Article Snippet: .. Seventytwo hours posttransfection, cells were harvested, and total cell lysate was extracted using IP lysis buffer (Pierce), followed by immunoprecipitation of FLAG-Mcl-1 using EZview anti-FlagM2Agarose (Sigma) overnight, and eluted by 3x FLAG peptide (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. This was followed by a second immunoprecipitation using HA beads (Invitrogen) to isolate ubiquitinated FLAG-Mcl-1 as per manufacturer’s protocol.

Article Title: Copper as a Novel Regulator of PERK
Article Snippet: .. The protein was eluted using 2mL 1.25mg/mL 3X Flag Peptide from Thermofisher in lysis buffer. ..

Immunoprecipitation:

Purification:

Article Title: Structural Basis for pre-tRNA Recognition and Processing by the Human tRNA Splicing Endonuclease Complex
Article Snippet: .. For BS3 crosslinking, CLP1 contained a C-terminal FLAG-Tag and the sample was purified using ANTI-FLAG M2 Affinity Gel (Sigma), eluted with Pierce 3X FLAG peptide (Thermo) and then further purified as described above for wt-TSEN. .. The TSEN•CLP1 (0.74 mg/mL) complex was crosslinked with 75 μM bis(sulfosuccinimidyl)suberate (BS3; Sigma).

Article Title: Structural basis for pre-tRNA recognition and processing by the human tRNA splicing endonuclease complex.
Article Snippet: .. For BS3 crosslinking, CLP1 contained a C-terminal FLAG-Tag, and the sample was purified using ANTI-FLAG M2 Affinity Gel (Sigma), eluted with Pierce 3X FLAG peptide (Thermo) and then further purified as described above for wt-TSEN. .. The TSEN–CLP1 (0.74 mg ml–1) complex was crosslinked with 75 μM bis(sulfosuccinimidyl)suberate (BS3; Sigma).

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